|
|
|
20th EDRA Webinar - June 30th, 202620th EDRA Webinar - June 30th, 2026, from 4:00 to 5:30 pm (CET)
Caroline Kisker - Keynote speaker "More than its parts? Component based analysis of TFIIH function" Rudolf Virchow Center for Integrative and Translational Bioimaging, Univ. of Würzburg, Würzburg, Germany The general transcription factor II H is a multiprotein molecular machine vital for two essential cellular processes, nucleotide excision repair (NER) and RNA polymerase II (Pol II) mediated transcription. Both processes require distinct features and activities that need to be accommodated within TFIIH. TFIIH is a ten-subunit complex that can be divided into core TFIIH (consisting of XPD, XPB, p62, p52, p44, p34, and p8) and the cyclin dependent kinase activating complex (CAK, consisting of CDK7, Cyclin H and MAT1), the latter being responsible for the effective initial phosphorylation of theI C-terminal domain of Pol II. We analysed key components of TFIIH ranging from small entities to full complexes revealing intricate interactions and regulations that define the activity of the complex. Our approach explores vital tasks including TFIIH translocation and damage verification, driven by the two superfamily motor proteins XPD and XPB, to CAK activity and specificity, surprisingly defined by the CAK component MAT1. |
